This document outlines procedures for the new regime of Material Transfer Agreements governing shipments of PGRFA to and from the Centres. The Guide will discuss first the rules governing the acquisition of PGRFA from countries and other collections, then deal with the transfers of Annex 1 PGRFA held by the CG Centres, and finally discuss the situation of non-Annex 1 PGRFA. The Guide has been prepared in consultation with Centres, members of the Executive Committee of the Inter-Centre Working Group on Genetic Resources and members of the Genetic Resources Policy Committee. It draws in particular on the work done by IRRI and Dr. Ruaraidh Sackville Hamilton. The Guide includes a section (Appendix 7) on Frequently Asked Questions on the Standard Material Transfer Agreement. The FAQs were updated in August 2010.